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SPERM PROCESSING

During coitus, the ejaculate is deposited around the cervix. Only progressively motile sperm having a normal morphology can penetrate and migrate through the cervical mucus.

In addition, there are factors in seminal plasma that actually inhibit fertilization (de capacitation factors), and prostaglandin. Although fertile sperm with progressive motility and normal morphology are able to pass through into the uterus, they can’t fertilize an egg until its seminal plasma coating are removed.

The sperm processing performed in the laboratory removes these harmful substances, along with dead or damaged sperm. The processing also concentrates all viable sperm into an amount ideally suited for Intra Uterine Insemination (IUI), a process by which a physician clinically places sperm within the uterus directly through the cervix.

CoitusIUI
Semen deposited inside the vaginaSperm deposited inside the uterus
  • Cervical mucus is a barrier
  • Cervical mucus barrier bypassed
  • Cervical mucus prevents entry of seminal plasma
  • Sperm washing removes seminal plasma
  • Prevents abnormal sperm, non-motile sperm and other cellular forms from entry
  • Sperm processing removes non-motile sperm and most of the abnormal sperm
  • Only few sperm in an ejaculate are able to enter cervical mucus
  • Almost all of the viable sperm in an ejaculate are deposited inside the uterus
  • Optimum time of IUI depends on any one or all of the following: Hormone levels, ultrasound measurement of your spouse’s follicles, urinary ovulation prediction kit response, and the sperm quality.

    Recommendations for Successful IUI outcome

    Double IUI utilizing the timing below:

    IUI Timing For Each Ovulation Detection Method
    IUIUrine LHHCG Injection
    FirstDay of Pos. Urine12 to 18 hrs post HCG
    SecondDay after Pos. Urine36 hrs post HCG

    Regardless, your physician will recommend the appropriate number of inseminations for your spouse based on her age, clinical findings and your sperm quality.

    We have developed a filter column which is marketed by Cook Ob/Gyn as “Sperm Filter Column”, has been shown to be very effective as compared to other procedures such as ‘Swim up” or Density Gradient Centrifugation procedures. The typical laboratory procedure requires about 20 minutes.

    Reference:
    Semen Collection and Processing Methods by R.S. Jeyendran
    Cambridge University Press, Cambridge, UK.